CLONE # :      

YNR-HMCAF
 

IMMUNOGEN :           

r.Human MCAF
 

Ig SUBCLASS :                           

Mouse IgG2a 
 

PURIFICATION METHOD:     

Protein A / S‑sepharose.
 

PROTEIN CONCENTRATION:      

1 mg/ml in PBS (after reconstitution).
 

STORAGE PROCEDURES:  

Lyophilized: store at 4^o C.  After reconstitution, if  not intended for use within a month, aliquot and   store at ‑20^o C.
 

RECONSTITUTION:

Reconstitute with 0.5 ml of H₂0.  Mix gently, wash  the sides of the vial and wait 30-60  seconds before use.
 

STABILITY / SHELF LIFE:

Two years lyophilized, 1month in solution at 4^oC.  
 

TITER:

 In direct ELISA, using alkaline phosphatase goat  anti-mouse Ig (Jackson Laboratories) 1:5,000 dilution will give 0.3 O.D within 10 minutes.
 

NOTE:

 MCAF does not bind to plastic as well as most proteins - use higher concentration for proper binding.

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