Human Follicle Stimulating Hormone

 ( HuFSH)

Certificate of Analysis and Data Sheet

 

       ×         Description:

Human FSH is a glycoprotein produced from urine of post-menopausal women and having a total molecular mass of 35,500 Dalton.

HuFSH is a heterodimeric hormone consisting of 165 amino acids a chain (non-active) and 115 amino acids b chain (active).

HuFSH induces maturation of Graafian follicles of the ovary, promotes development of germinal cells in males and activates cytosolic tyrosine kinase.

         The HuFSH is purified by proprietary chromatographic techniques.  

 

       ×         Source:

Urine of post-menopausal women

 

       ×          Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

 

       ×         Solubility:                                                                                                                                                        The lyophilized HuFSH is very soluble in water.

 

       ×         Stability:

The protein was lyophilized from a concentrated (1mg/ml) solution with no additives.

 

       ×         Purity:

Greater than 98.0% as determined by:

 (a) Analysis by RP-HPLC.

           (b) Anion-exchange FPLC.

         (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

 

(Limit of acceptance:³98.0%. No more than 2% total impurities; no single impurity greater than 1%)

 

 

       ×         Biological Activity:

Our HuFSH is biologically active compared to standard (IRP reference preparation 68/140).

  The activity was found to be 9780IU/mg.

 

       ×         Contaminents:

Less than: 0.1% hCG, 0.5%TSH, 0.5% LH, 0.5%GH and 0.5%Prl.

Free of HbsAg, antibodies to HIV and HCV. 

 

       ×         Protein content:

          Protein quantitation was carried out by two independent methods:

 

1. UV spectroscopy at 280 nm.

 

2.  Analysis by RP-HPLC, using a calibrated solution of FSH as a Reference Standard.

 

      ×         Usage:

This material is offered  for research, laboratory or further manufacturing purposes.