Human Growth Hormone Releasing Hormone

(HuGHRH)

Certificate of Analysis and Data Sheet

       ×         Description:

Synthetic Human GHRH is a single, non-glycosylated, polypeptide chain containing 29 amino acids and having a molecular mass of  3358 Dalton.

         Corresponds to the amino-terminal segment of the naturally occurring human growth hormone-releasing hormone consisting of 44 amino acid residues.

         

       ×         Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

 

       ×         Formulation & Packaging:

The protein (1mg/ml) was lyophilized after extensive dialyses against 1.7mg sodium phosphate buffer (0.1 mg sodium phosphate monobasic & 1.6mg sodium phosphate dibasic).      

 

       ×         Solubility:

The lyophilized HuGHRH is very soluble in water and most aqueous buffers.

The reconstituted solution has a pH of 5.0 to 5.5.

 

       ×         Stability:

Lyophilized HuGHRH although stable at room temperature, should be stored desiccated below 0°C. Reconstituted HuGHRH  is best stored refrigerated at 4°C.

 

       ×         Purity:

Greater than 99.0% as determined by:

 (a) Analysis by RP-HPLC.

         (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

 

(Limit of acceptance:³98.0%. No more than 2% total impurities; no single impurity greater than 1%)

 

 

       ×         Amino Acid Composition:

          The free base of sermorelin has the empirical formula C 149 H 246 N 44 O 42 S.

           Tyr-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg-Lys-Val-Leu-Gly-Gln-Leu-Ser-Ala-Arg-Lys-Leu-Leu-Gln-Asp-Ile-Met-Ser-Arg-NH2

 

       ×         Amino acid sequence:

The sequence of the first five N-terminal amino acids was determined and was found to be Tyr-Ala-Asp-Ala-Ile, conforming to the sequence.

 

       ×         Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

 

       ×         Biological Activity:

Our HuGHRH is fully biologically active, increases plasma growth hormone concentrations by directly stimulating the anterior pituitary gland to release natural human growth hormone.

 

       ×         Endotoxin:

Less than 0.1 ng/µg (IEU/µg) of HuGHRH.

 

       ×         Protein content:

          Protein quantitation was carried out by two independent methods:

 

1. UV spectroscopy at 280 nm

 

2.  Analysis by RP-HPLC, using a calibrated solution of GHRH as a Reference Standard.

 

      ×         Usage:

This material is offered  for research, laboratory or further manufacturing purposes.