Recombinant HIV-I Antigen
Certificate of Analysis
and Data Sheet
Description:
This
antigen is an E.coli-derived recombinant protein that composes all of the
reported immunogenic determinants found in gp41 and a small portion of gp120. The
gene encoding this fusion protein was synthesized using codons optimized for
E.coli expression and doesn’t represent a linear HIV-I envelope sequence.
rHIV-I
is a non-glycosylated, polypeptide
chain, having a molecular mass of 27,333
The rHIV-I is purified by standard chromatographic
techniques.
Source:
Escherichia Coli.
Physical Appearance:
Sterile
Filtered White lyophilized (freeze-dried)
powder.
Formulation & packaging:
The protein was lyophilized after extensive
dialysis against 5mM phosphate buffer pH=7.3 containing 0.01% SDS.
Solubility:
The lyophilized rHIV-I is soluble in water and most aqueous buffers below and above the isoelectric point (pI=9.7).
Purity:
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE
(Limit of acceptance:³98.0%. No more than 2% total
impurities; no single impurity greater than 1%)
Stability:
Lyophilized rHIV-I, although
stable at room temperature, should be stored desiccated below 0°C. Reconstituted rHIV-I is best stored refrigerated at 4°C.
Biological Activity:
This antigen was thoroughly tested in various tests as
well as ELISA using the standard BBI serum panels.
All antibody detection tests on this antigen were as
sensitive or more than the standard Abott third generation tests.
Protein content: Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 2.94 as the extinction coefficient
for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences
(IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of HIV-I as a Reference Standard.
Usage: This material is offered for research, laboratory or further manufacturing purposes.
Send mail to:
customer_service@abazyme.com with questions or comments.
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