Recombinant Human Hepatocyte Growth Factor

 ( rHuHGF)

Certificate of Analysis and Data Sheet

 

       ×         Description:

Recombinant Human HGF produced in Baculovirus is a heterodimer, non-glycosylated, polypeptide chain consisting an a-chain of 463 amino acids and b-chain of 234 having a total molecular mass of 80017 Dalton.

         The rHuHGF is purified by proprietary chromatographic techniques.

 

       ×         Source:

Baculovirus Infected High-5 cells.

 

       ×         Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

 

       ×         Formulation & Packaging:

The protein was lyophilized from a concentrated (1mg/ml) solution containing 0.1M

L-Arginine and 10mM Tris at pH=8.

 

       ×         Solubility:

The lyophilized rHuHGF is very soluble in water and most aqueous buffers below and above the isoelectric point.

 

       ×         Stability:

Lyophilized rHuHGF although stable at room temperature, should be stored desiccated below 0°C. Reconstituted rHuHGF is best stored refrigerated at 4°C.

 

       ×         Purity:

Greater than 98.0% as determined by:

 (a) Analysis by RP-HPLC.

           (b) Anion-exchange FPLC.

         (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

(Limit of acceptance:³98.0%. No more than 2% total impurities; no single impurity greater than 1%)

 

       ×         Amino Acid Composition:

In total agreement with the expected amino acid composition of native human HGF .

 

       ×         Amino acid sequence:

Agrees with the sequence of native human HGF.

 

       ×         Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

 

       ×         Biological Activity:

Our rHuHGF is fully biologically active when compared to standards.

  The ED50,  calculated by the dose-dependant proliferation of monkey MBr-5 indicator cells was found to be 20-40 ng/ml.

 

       ×         Endotoxin:

Less than 0.1 ng/µg (IEU/µg) of rHuHGF.

 

       ×         Protein content:

          Protein quantitation was carried out by two independent methods:

 

1.  UV spectroscopy at 280 nm.

 

2.  Analysis by RP-HPLC, using a calibrated solution of HGF as a Reference Standard.

 

      ×        Usage:

This material is offered for research, laboratory or further manufacturing purposes.