-cm H2O not
less than 100µg/ml, which can then be further diluted to other
aqueous solutions. Recombinant Human Insulin-Like Growth Factor II
Certificate of Analysis and Data Sheet
Description: Recombinant Human IGF-II produced in E.Coli
is a single, non-glycosylated, polypeptide chain
containing 67 amino acids and having a molecular mass of
7505 Dalton.
rHuIGF-II is purified by
proprietary chromatographic techniques.
Source: Escherichia Coli.
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation & Packaging: The protein was lyophilized with no additives.
Solubility: It is recommended to
reconstitute the lyophilized rHuIGF-II in sterile 18M-cm H2O not
less than 100µg/ml, which can then be further diluted to other
aqueous solutions.
Stability: Lyophilized rHuIGF-II
although stable at room temperature for 3 weeks, should be
stored desiccated below -18
C. Upon reconstitution
rHuIGF-II should be stored at 4 C between 2-7 days and
for future use below -18 C.
For long term
storage it is recommended to add a carrier protein (0.1% HSA
or BSA).
Please prevent freeze-thaw cycles.
Purity: Greater than 97.0% as determined
by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange
FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE
Silver Stained gel.
Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Tyr-Arg-Pro-Ser.
Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological Activity:
Our rHuIGF-II
is fully biologically active when compared to
standards.
The ED50, calculated by the competitive binding
of IGF-II plasma derived to human placental membrane is <
1.0 ng/ml, corresponding to a specific activity of 10
U/mg.
Endotoxin: Less than 0.1 ng/µg (IEU/µg) of rHuIGF-II.
Protein content: Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC, using a calibrated solution of IGF-II as a Reference Standard.
Usage: This material is offered for research, laboratory or further manufacturing purposes.
Send e-mail to:
customer_service@abazyme.com with questions or comments.
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