Recombinant Human Stromal
Cell-Derived Factor-1 alpha

(rHuSDF-1a)

Certificate of Analysis and Data Sheet

       ×         Description:

Recombinant Human IL-SDF-1a produced in E.Coli is a non-glycosylated, Polypeptide chain containing 68 amino acids and having a molecular mass of 8008 Dalton.

         The rHuSDF-1a is purified by proprietary chromatographic techniques.

 

       ×         Source:

Escherichia Coli.

 

       ×          Physical Appearance:

Sterile Filtered White lyophilized (freeze-dried) powder.

 

       ×         Formulation:

The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives.

 

       ×         Solubility:

It is recommended to reconstitute the lyophilized rHuSDF-1a in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

 

       ×         Stability:

Lyophilized rHuSDF-1a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuSDF-1a should be stored  at 4°C between 2-7 days and for future use below -18°C.

For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent freeze-thaw cycles.

 

       ×         Purity:

Greater than 99.0% as determined by:

(a) Analysis by RP-HPLC.

(b) Anion-exchange FPLC.

     (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

 

 

       ×         Amino acid sequence:

The sequence of the first five N-terminal amino acids was determined and was found to be Lys-Pro-Val-Ser-Leu

 

       ×         Dimers and aggregates:

Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

 

       ×         Biological Activity:

Our SDF-1a is fully biologically active when compared to standard.

The specific activity as determined by its ability to chemoattract human peripheral T cells activated with PHA and IL-2 using a concentation of 20-80 ng/ml.

 

       ×         Endotoxin:

Less than 0.1 ng/µg (IEU/µg) of SDF-1a.

 

       ×         Protein content:

 Protein quantitation was carried out by two independent methods:

 

1.    UV spectroscopy at 280 nm.

 

2.    Analysis by RP-HPLC, using a standard solution of  SDF-1a as a Reference Standard.

 

      ×         Usage:

This material is offered for research, laboratory or further manufacturing purposes.